3EG5
Crystal structure of MDIA1-TSH GBD-FH3 in complex with CDC42-GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 |
| Unit cell lengths | 71.269, 71.269, 244.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.470 - 2.700 |
| R-factor | 0.20816 |
| Rwork | 0.206 |
| R-free | 0.24333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.478 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.074 | 0.381 |
| Number of reflections | 38038 | |
| <I/σ(I)> | 13.52 | 3.69 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | Bis-Tris-Propane (PH 8.8 adjusted with citric acid), 26% (w/v) PEG 3350, 250mM Na-Tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
