3E7O
Crystal Structure of JNK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.688, 78.784, 78.927 |
| Unit cell angles | 90.00, 96.43, 90.00 |
Refinement procedure
| Resolution | 44.290 - 2.140 |
| R-factor | 0.21868 |
| Rwork | 0.217 |
| R-free | 0.25031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jnk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.111 | 0.468 |
| Number of reflections | 51687 | |
| <I/σ(I)> | 8.4 | 1.9 |
| Completeness [%] | 96.7 | 84 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 0.1M MES, 0.6M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
