3DZA
Crystal structure of a putative membrane protein of unknown function (yfdx) from klebsiella pneumoniae subsp. at 1.65 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97937,0.91837,0.97874 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.207, 79.016, 137.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.975 - 1.650 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.19400 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.387 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.975 | 29.980 | 1.690 |
| High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
| Rmerge | 0.110 | 0.044 | 0.618 |
| Total number of observations | 3963 | 25665 | |
| Number of reflections | 90273 | ||
| <I/σ(I)> | 6.1 | 12.7 | 1.2 |
| Completeness [%] | 99.9 | 98.1 | 99.8 |
| Redundancy | 3.9 | 3.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 20.0000% PEG-3350, 0.2000M ZnAcetate, No Buffer pH 6.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
