3DXT

Crystal structure of the catalytic core domain of JMJD2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	130
Collection date	2007-02-28
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	71.630, 71.630, 151.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.240 - 1.800
R-factor	0.17554
Rwork	0.174
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	jmjd2a
RMSD bond length	0.016
RMSD bond angle	1.493
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Number of reflections	34305

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	hepes, 2.0M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K