3DRC

INVESTIGATION OF THE FUNCTIONAL ROLE OF TRYPTOPHAN-22 IN ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE BY SITE-DIRECTED MUTAGENESIS

Experimental procedure

Spacegroup name	P 61
Unit cell lengths	93.120, 93.120, 73.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.156
RMSD bond length	0.022
RMSD bond angle	2.990
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.049 *
Total number of observations	30609 *
Completeness [%]	96.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MTX	60 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	drop	histidine	0.05 (M)
4	1	drop	calcium acetate	0.05 (M)
5	1	reservoir	ethanol	23 (%)
6	1	reservoir	Tris	0.1 (M)