3DPL

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-08
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	62.965, 65.510, 141.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.244
Rwork	0.244
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.382
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.156	0.407
Number of reflections	18719
<I/σ(I)>	16.1	2
Completeness [%]	95.5
Redundancy	5.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	2% PEG 3350, 0.1M HEPES, 0.2M L-proline, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K