3DOX
X-ray structure of HIV-1 protease in situ product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97644 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.380, 62.380, 82.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.110 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23600 |
| RMSD bond length | 0.006 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.080 |
| Number of reflections | 12068 |
| <I/σ(I)> | 18.03 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 300 | 1-5% saturated ammonium sulfate, pH 6.2, Soaking, temperature 300.0K |
