3D94
Crystal structure of the insulin-like growth factor-1 receptor kinase in complex with PQIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 208.182, 208.182, 50.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.650 - 2.300 |
| R-factor | 0.19997 |
| Rwork | 0.198 |
| R-free | 0.23621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.394 |
| Number of reflections | 17319 | |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 97.5 | 99.2 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 12% PEG8000, 0.1 M imidazole, pH 7.5 and 0.2 M calcium acetate, VAPOR DIFFUSION, temperature 293K |
