3D8V
Crystal structure of GlmU from Mycobacterium tuberculosis in complex with uridine-diphosphate-N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.238, 114.238, 361.796 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.979 - 2.550 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hm9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.067 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.490 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.110 | 0.768 |
| Number of reflections | 31984 | |
| <I/σ(I)> | 22.3 | 2.3 |
| Completeness [%] | 100 | |
| Redundancy | 11.8 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 5% PEG 8000, 0.1 M Cacodylate, 9% MPD, 12% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
