3D8V

Crystal structure of GlmU from Mycobacterium tuberculosis in complex with uridine-diphosphate-N-acetylglucosamine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0000902	biological_process	cell morphogenesis
A	0003824	molecular_function	catalytic activity
A	0003977	molecular_function	UDP-N-acetylglucosamine diphosphorylase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
A	0008360	biological_process	regulation of cell shape
A	0009245	biological_process	lipid A biosynthetic process
A	0009252	biological_process	peptidoglycan biosynthetic process
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0019134	molecular_function	glucosamine-1-phosphate N-acetyltransferase activity
A	0035635	biological_process	entry of bacterium into host cell
A	0044650	biological_process	adhesion of symbiont to host cell
A	0046872	molecular_function	metal ion binding
A	0070569	molecular_function	uridylyltransferase activity
A	0071555	biological_process	cell wall organization

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE UD1 A 496

Chain	Residue
A	LEU12
A	SER112
A	TYR150
A	GLY151
A	GLU166
A	ASN181
A	THR211
A	ASN239
A	HOH497
A	HOH517
A	HOH586
A	ALA14
A	GLY15
A	GLN83
A	PRO86
A	LEU87
A	GLY88
A	THR89
A	ALA92

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	239
Details	Region: {"description":"Pyrophosphorylase","evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Region: {"description":"Linker","evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19237750","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23485416","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2012","submissionDatabase":"PDB data bank","title":"Structural snapshots of Glmu from Mycobacterium tuberculosis.","authors":["Jagtap P.A.","Verma S.K.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2012","submissionDatabase":"PDB data bank","title":"Structural snapshots of Glmu from Mycobacterium tuberculosis.","authors":["Jagtap P.A.","Verma S.K.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19237750","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19237750","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23485416","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2012","submissionDatabase":"PDB data bank","title":"Structural snapshots of Glmu from Mycobacterium tuberculosis.","authors":["Jagtap P.A.","Verma S.K.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19121323","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23485416","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}},{"source":"PDB","id":"3SPT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4HCQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23485416","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2012","submissionDatabase":"PDB data bank","title":"Structural snapshots of Glmu from Mycobacterium tuberculosis.","authors":["Jagtap P.A.","Verma S.K.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01631","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUL-2011","submissionDatabase":"PDB data bank","title":"Structure of Mycobacterium tuberculosis GlmU in complex with Acetyl CoA.","authors":["Jagtap P.A.","Prakash B."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Modified residue: {"description":"N-acetylthreonine","evidences":[{"source":"PubMed","id":"21969609","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Cross-link: {"description":"Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)","evidences":[{"source":"PubMed","id":"20066036","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1lxa

Chain	Residue	Details
A	GLU385

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1lxa

Chain	Residue	Details
A	ARG19

---