3D87
Crystal structure of Interleukin-23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.098, 240.587, 141.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.390 - 2.900 |
| R-factor | 0.2687 |
| Rwork | 0.268 |
| R-free | 0.32320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d85 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.390 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.577 |
| Number of reflections | 41680 | |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 93.5 | 10 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 1.26M K2HPO4, 0.5M NaH2PO4, 100 mM Imidazole, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
