3D87
Crystal structure of Interleukin-23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.098, 240.587, 141.781 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.390 - 2.900 |
R-factor | 0.2687 |
Rwork | 0.268 |
R-free | 0.32320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d85 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.516 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.390 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.577 |
Number of reflections | 41680 | |
<I/σ(I)> | 9.8 | 2.1 |
Completeness [%] | 93.5 | 10 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 1.26M K2HPO4, 0.5M NaH2PO4, 100 mM Imidazole, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |