3D1M
Crystal Structure of Sonic Hedgehog Bound to the third FNIII domain of CDO
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Detector technology | CCD |
| Collection date | 2007-08-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97893 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.179, 98.582, 144.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.560 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.416 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.471 |
| Number of reflections | 55705 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.0 | 91.1 |
| Redundancy | 6.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 28% PEG 3350, 200 mM MgCl2, 100 mM Tris pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
