3D12
Crystal Structures of Nipah Virus G Attachment Glycoprotein in Complex with its Receptor Ephrin-B3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Collection date | 2007-10-30 |
Wavelength(s) | 0.9192 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 189.492, 189.492, 277.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.337 - 3.005 |
Rwork | 0.221 |
R-free | 0.26500 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.927 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.086 | 0.029 | 0.469 |
Number of reflections | 58204 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 98.9 | 98.6 | 98.1 |
Redundancy | 3.6 | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5M Li2SO4 and 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |