3CYY
The crystal structure of ZO-1 PDZ2 in complex with the Cx43 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-04 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 41.040, 68.140, 149.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.21715 |
Rwork | 0.215 |
R-free | 0.25366 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | synaptojanin-2 binding protein (PDB id: 2JIN) and PICK1 (PDB id: 2GZV) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.216 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.160 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.051 |
Number of reflections | 8265 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M CaCl2, 0.1M HEPES, 14%(w/v) polyethylene glycol 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |