3CTJ
Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-met in complex with a aminopyridine based inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-17 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.813, 47.106, 153.422 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.2091 |
Rwork | 0.206 |
R-free | 0.26110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.886 |
Data reduction software | HKL-2000 ((DENZO)) |
Data scaling software | HKL-2000 ((SCALEPACK)) |
Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.104 | 0.297 |
Number of reflections | 10367 | |
<I/σ(I)> | 17.2 | 10.1 |
Completeness [%] | 91.3 | 95.2 |
Redundancy | 10.4 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.1 | 12% MEPEG 5000, 0.1M HEPES (PH 7.1) 11% 2-PROPANOL |