3CPW
The structure of the antibiotic LINEZOLID bound to the large ribosomal subunit of HALOARCULA MARISMORTUI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-10-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.730, 298.580, 575.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
Rwork | 0.191 |
R-free | 0.23000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNX |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.054 | 0.412 |
Number of reflections | 470299 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 95.6 | 84.2 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MGCl2 | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 2 | MGCl2 | ||
4 | 1 | 2 | KCL | ||
5 | 1 | 2 | NH4CL | ||
6 | 1 | 2 | PEG6000 |