3COA

Crystal Structure of FoxO1 DBD Bound to IRE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.88560
Spacegroup name	P 21 21 2
Unit cell lengths	76.062, 102.432, 65.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.216
R-free	0.25000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.064	0.037	0.327
Number of reflections	49316
<I/σ(I)>	10.6
Completeness [%]	99.4	99.5	98.1
Redundancy	11	10.3	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	30% PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	2	PEG 4000