3CMD
Crystal structure of peptide deformylase from VRE-E.faecium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 149.470, 149.470, 143.329 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.22900 |
Starting model (for MR) | 1lm6 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (within the CCP4 program suite) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 25754 | |
<I/σ(I)> | 12.2 | 3.45 |
Completeness [%] | 75.0 | 97.4 |
Redundancy | 7.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | Na-malonate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |