3CIY
Mouse Toll-like receptor 3 ectodomain complexed with double-stranded RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2007-11-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.846, 225.877, 259.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.290 - 3.410 |
| R-factor | 0.288 |
| Rwork | 0.288 |
| R-free | 0.33300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cig |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.110 | 0.514 |
| Number of reflections | 46438 | |
| <I/σ(I)> | 17.4 | 1.81 |
| Completeness [%] | 97.8 | 95.3 |
| Redundancy | 4.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 13% PEG 3350, 5% Dextran 5000, 0.2M Ammonium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | Dextran 5000 | ||
| 3 | 1 | 1 | Ammonium Citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | Dextran 5000 | ||
| 7 | 1 | 2 | Ammonium Citrate | ||
| 8 | 1 | 2 | H2O |
