3CFR
Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-02 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.958, 119.769, 130.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.19577 |
Rwork | 0.192 |
R-free | 0.26584 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ig9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.337 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.187 | |
Number of reflections | 46306 | |
Completeness [%] | 99.7 | |
Redundancy | 6.8 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 7% PEG350 monomethyl ether, 0.1M NA CACODYLATE, 0.1 M CA2CL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NA CACODYLATE | ||
2 | 1 | 1 | CA2CL2 | ||
3 | 1 | 2 | NA CACODYLATE | ||
4 | 1 | 2 | CA2CL2 | ||
5 | 1 | 2 | PEG350 monomethyl ether |