3CDZ
Crystal structure of human factor VIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.113, 134.113, 349.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.980 |
R-factor | 0.259 |
Rwork | 0.256 |
R-free | 0.32700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology models of individual domain built by SWISS-MODEL using PDB entries 1SDD 1KCW and 1D7P. |
RMSD bond length | 0.014 |
RMSD bond angle | 1.735 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.980 | 3.980 |
Rmerge | 0.130 | 0.827 |
Number of reflections | 26444 | |
<I/σ(I)> | 27.2 | 1.7 |
Completeness [%] | 99.0 | 92.3 |
Redundancy | 13.2 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris-HCl, 10% EtOH, 7% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |