3CDW
Crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase (3Dpol) in complex with protein primer VPg and a pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.213, 74.213, 285.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.450 - 2.500 |
| R-factor | 0.1829 |
| Rwork | 0.175 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cdu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.041 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (2.3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.450 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 28791 | |
| <I/σ(I)> | 17 | 2.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.45 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 2M ammonium sulfate, 0.1M CAPS, 0.2M lithium sulfate, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
