3CBL
Crystal structure of human feline sarcoma viral oncogene homologue (v-FES) in complex with staurosporine and a consensus peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-01-25 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.679, 77.178, 149.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.580 - 1.750 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bkb |
RMSD bond length | 0.014 |
RMSD bond angle | 1.591 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.069 | 0.541 |
Number of reflections | 42378 | |
<I/σ(I)> | 12.3 | 2 |
Completeness [%] | 99.3 | 100 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% PEG 3350, 0.1M Na Malate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |