3C5F

Structure of a binary complex of the R517A Pol lambda mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-21
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	94.237, 151.884, 85.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
Rwork	0.224
R-free	0.26300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.113	0.066	0.606
Number of reflections	56848
<I/σ(I)>	11.3
Completeness [%]	96.8	99.7	91.5
Redundancy	5.6	7.8	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2M ammonium acetate, 100 mM Hepes, pH 7 and 10% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium acetate
2	1	1	Hepes
3	1	1	PEG 4000
4	1	2	ammonium acetate
5	1	2	Hepes
6	1	2	PEG 4000