3C5F
Structure of a binary complex of the R517A Pol lambda mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.237, 151.884, 85.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| Rwork | 0.224 |
| R-free | 0.26300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
| Rmerge | 0.113 | 0.066 | 0.606 |
| Number of reflections | 56848 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 96.8 | 99.7 | 91.5 |
| Redundancy | 5.6 | 7.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M ammonium acetate, 100 mM Hepes, pH 7 and 10% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | ammonium acetate | ||
| 5 | 1 | 2 | Hepes | ||
| 6 | 1 | 2 | PEG 4000 |
