3C5C
Crystal structure of human Ras-like, family 12 protein in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-12-18 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 34.040, 66.436, 71.943 |
Unit cell angles | 101.46, 90.03, 89.89 |
Refinement procedure
Resolution | 30.000 - 1.850 |
R-factor | 0.203 |
Rwork | 0.202 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2atv |
RMSD bond length | 0.014 |
RMSD bond angle | 1.329 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.3.0037) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
Rmerge | 0.119 | 0.041 | 0.955 |
Number of reflections | 52667 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 98.9 | 99.9 | 95.6 |
Redundancy | 4.3 | 4.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 0.1 M Tris-HCl pH 8.5, 28% PEG 4000, 0.2 M Magnesium chloride. In situ proteolysis with 1:100 endoproteinase Glu-C V8, VAPOR DIFFUSION, temperature 291K |