3C5C

Crystal structure of human Ras-like, family 12 protein in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-12-18
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	34.040, 66.436, 71.943
Unit cell angles	101.46, 90.03, 89.89

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.203
Rwork	0.202
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2atv
RMSD bond length	0.014
RMSD bond angle	1.329
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.119	0.041	0.955
Number of reflections	52667
<I/σ(I)>	7.1
Completeness [%]	98.9	99.9	95.6
Redundancy	4.3	4.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	0.1 M Tris-HCl pH 8.5, 28% PEG 4000, 0.2 M Magnesium chloride. In situ proteolysis with 1:100 endoproteinase Glu-C V8, VAPOR DIFFUSION, temperature 291K