3C59
Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9782, 0.9790, 0.96 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.850, 75.850, 87.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.930 - 2.300 |
R-factor | 0.19869 |
Rwork | 0.196 |
R-free | 0.24080 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.994 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.074 | 0.561 |
Number of reflections | 13273 | |
<I/σ(I)> | 19 | 4.05 |
Completeness [%] | 99.4 | 98.9 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 0.1M MgCl2, 0.4M MgTartrate, 9mM n-Decyl-beta-D-thiomaltoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |