3BZJ
UVDE K229L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-16 |
| Detector | MAR225 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 113.390, 113.390, 89.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.280 - 2.300 |
| R-factor | 0.19042 |
| Rwork | 0.188 |
| R-free | 0.24414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6v |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.367 |
| Number of reflections | 15387 | |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 11.3 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | Ammoniumsulfate, Acetate, Lithiumsulfate, pH6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
