2J6V

Crystal structure of the DNA repair enzyme UV Damage Endonuclease

Summary for 2J6V

• Entry DOI: 10.2210/pdb2j6v/pdb
• Descriptor: UV ENDONUCLEASE, MANGANESE (II) ION, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: uvde, plasmid, tim barrel, dna repair, endonuclease, dna binding protein, lyase
• Biological source: THERMUS THERMOPHILUS
• Total number of polymer chains: 2
• Total formula weight: 68944.09

Authors

Paspaleva, K.,Thomassen, E.A.J.,Pannu, N.S.,Goossen, N.,Abrahams, J.P. (deposition date: 2006-10-04, release date: 2007-10-16, Last modification date: 2024-10-23)

Primary citation

Paspaleva, K.,Thomassen, E.A.J.,Pannu, N.S.,Iwai, S.,Moolenaar, G.F.,Goosen, N.,Abrahams, J.P.
Crystal Structure of the DNA Repair Enzyme Ultraviolet Damage Endonuclease.
Structure, 15:1316-, 2007

PubMed Abstract: The ultraviolet damage endonuclease (UVDE) performs the initial step in an alternative excision repair pathway of UV-induced DNA damage, nicking immediately adjacent to the 5' phosphate of the damaged nucleotides. Unique for a single-protein DNA repair endonuclease, it can detect different types of damage. Here we show that Thermus thermophilus UVDE shares some essential structural features with Endo IV, an enzyme from the base excision repair pathway that exclusively nicks at abasic sites. A comparison between the structures indicates how DNA is bound by UVDE, how UVDE may recognize damage, and which of its residues are involved in catalysis. Furthermore, the comparison suggests an elegant explanation of UVDE's potential to recognize different types of damage. Incision assays including point mutants of UVDE confirmed the relevance of these conclusions.

PubMed: 17937920
DOI: 10.1016/J.STR.2007.05.010

Experimental method

X-RAY DIFFRACTION (1.55 Å)