3BWN
L-tryptophan aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.410, 97.830, 139.460 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 29.660 - 2.250 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.25300 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 1lk9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.734 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 110711 | |
| <I/σ(I)> | 12.5 | 2.53 |
| Completeness [%] | 90.0 | 82.6 |
| Redundancy | 2.49 | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 13-19% PEG 3350, 0.3M KCl, 100mM MOPSO pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K, pH 7.00 |
