3BUK
Crystal Structure of the Neurotrophin-3 and p75NTR Symmetrical Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2007-12-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0030 |
| Spacegroup name | H 3 |
| Unit cell lengths | 125.800, 125.800, 133.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.22749 |
| Rwork | 0.224 |
| R-free | 0.28771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nt3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.725 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.381 |
| Number of reflections | 23326 | |
| Completeness [%] | 96.2 | 97.3 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 290 | 0.9M Lithium Sulfate, 0.05M Na Citrate, 0.7M Ammonium Sulfate, pH 5.0, EVAPORATION, temperature 290K |
