3BSQ
Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 113.580, 113.580, 326.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.217 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1npm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.154 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.390 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.162 | 0.415 |
| Number of reflections | 19524 | |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 3.2M AMMONIUM SULFATE, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
