3BN3
crystal structure of ICAM-5 in complex with aL I domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.793, 71.507, 143.871 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.980 - 2.099 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23500 |
| Starting model (for MR) | PDB IDS 1T0P AND 1IC1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0026) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.980 | 2.180 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.072 | 0.454 |
| Number of reflections | 37303 | |
| <I/σ(I)> | 3.76 | |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 8.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1M HEPES pH7.5, 7.5%PEG8000, 10%glycerol, 5mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
