3BLB
Crystal structure of Golgi Mannosidase II in complex with swainsonine at 1.3 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.767, 109.662, 138.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 1.300 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hww |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.112 | 0.654 |
| Number of reflections | 254722 | |
| <I/σ(I)> | 14.04 | 2.34 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 5.94 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tris-HCl, PEG 6000, MPD, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
