3BHH
Crystal structure of human calcium/calmodulin-dependent protein kinase IIB isoform 1 (CAMK2B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97649 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.400, 79.200, 98.700 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 47.780 - 2.400 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v7o |
RMSD bond length | 0.017 |
RMSD bond angle | 1.562 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.780 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.116 | 0.443 |
Number of reflections | 50690 | |
<I/σ(I)> | 9.77 | 2.69 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 3.76 | 3.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 50% PEG 300, 0.2M NaCl, 0.1M Na/KPO4 pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |