3B6F
Nucleosome core particle treated with cisplatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2007-02-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.072 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.327, 109.380, 180.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.660 - 3.450 |
R-factor | 0.331 |
Rwork | 0.330 |
R-free | 0.40200 |
Starting model (for MR) | 1kx5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.245 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 93.660 | 109.380 | 3.640 |
High resolution limit [Å] | 3.450 | 10.910 | 3.450 |
Rmerge | 0.076 | 0.047 | 0.485 |
Total number of observations | 4189 | 30581 | |
Number of reflections | 28162 | ||
<I/σ(I)> | 5.5 | 13.7 | 1.4 |
Completeness [%] | 99.1 | 74.8 | 100 |
Redundancy | 7.3 | 5.6 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | MnCl2, KCl, K-Cacodylate, pH 6.0, vapor diffusion, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MnCl2 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | K-Cacodylate | ||
4 | 1 | 2 | MnCl2 | ||
5 | 1 | 2 | KCl | ||
6 | 1 | 2 | K-Cacodylate |