3AM7
Crystal structure of the ternary complex of eIF4E-M7GTP-4EBP2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 87.840, 87.840, 37.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.200 |
| Rwork | 0.230 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.685 |
| RMSD bond angle | 2.500 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 43.900 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.101 | 0.101 |
| Number of reflections | 16434 | |
| <I/σ(I)> | 8 | 8 |
| Completeness [%] | 95.4 | 95.4 |
| Redundancy | 3.81 | 3.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 20% PEG-MME 2000, 0.1M MES, 0.2M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
