3AL6
Crystal structure of Human TYW5
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.48505 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 165.871, 165.871, 105.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.017 - 2.800 |
R-factor | 0.2427 |
Rwork | 0.240 |
R-free | 0.29540 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.082 | 0.050 | 0.480 |
Number of reflections | 36950 | ||
<I/σ(I)> | 56.444 | ||
Completeness [%] | 99.6 | 95.8 | 98.1 |
Redundancy | 19 | 22 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200mM potassium thiocyanate, 10% (w/v) PEG3350, pH 8.0, vapor diffusion, sitting drop, temperature 293K |