3AC9
Crystal structure of human NUDT5 complexed with 8-oxo-dGDP and manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.240, 40.442, 99.418 |
| Unit cell angles | 90.00, 121.59, 90.00 |
Refinement procedure
| Resolution | 28.269 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l85 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.368 |
| Number of reflections | 22415 | |
| <I/σ(I)> | 31.3 | 3.4 |
| Completeness [%] | 97.6 | 87.7 |
| Redundancy | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.1M Sodium Citrate, 35% PEG3350, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
