3A4U
Crystal structure of MCFD2 in complex with carbohydrate recognition domain of ERGIC-53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.410, 59.410, 198.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.810 - 1.840 |
| R-factor | 0.18828 |
| Rwork | 0.188 |
| R-free | 0.20042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.086 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.810 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.059 | 0.257 |
| Number of reflections | 36530 | |
| <I/σ(I)> | 19.5 | 6.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 20% PEG 3350, 0.2M sodium tartrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
