3A1B
Crystal structure of the DNMT3A ADD domain in complex with histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.547, 68.547, 109.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.720 - 2.292 |
| R-factor | 0.19344 |
| Rwork | 0.192 |
| R-free | 0.22408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a1a |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.721 | 2.380 |
| High resolution limit [Å] | 2.292 | 2.292 |
| Rmerge | 0.082 | 0.428 |
| Number of reflections | 12159 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 98.3 | 86.9 |
| Redundancy | 24.1 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% PEG 2000 monomethyl ether, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
