3ZU7
Crystal structure of a designed selected Ankyrin Repeat protein in complex with the MAP kinase ERK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-12 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.040, 89.370, 99.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.430 - 1.970 |
| R-factor | 0.22113 |
| Rwork | 0.219 |
| R-free | 0.26928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ERK 1mj0 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.957 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.410 | 1.880 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 42412 | |
| <I/σ(I)> | 19.21 | 8.3 |
| Completeness [%] | 82.9 | 44.8 |
| Redundancy | 5.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS-HOAC (PH 8.5), 20% PEG4K, 5 MM CDCL2 |
