3ZJY

Crystal Structure of Importin 13 - RanGTP - eIF1A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-28
Detector	DECTRIS PILATUS
Spacegroup name	C 1 2 1
Unit cell lengths	186.500, 100.400, 274.720
Unit cell angles	90.00, 90.91, 90.00

Refinement procedure

Resolution	49.549 - 3.600
R-factor	0.2579
Rwork	0.256
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x19
RMSD bond length	0.003
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (MR)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.700
High resolution limit [Å]	3.600	3.600
Rmerge	0.080	0.920
Number of reflections	57266
<I/σ(I)>	11.35	2.08
Completeness [%]	96.6	87.4
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1