3ZIU
Crystal structure of Mycoplasma mobile Leucyl-tRNA Synthetase with Leu-AMS in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 202.851, 202.851, 213.922 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 135.760 - 2.070 |
| R-factor | 0.16155 |
| Rwork | 0.160 |
| R-free | 0.19893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aq7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.120 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.230 | 0.990 |
| Number of reflections | 101516 | |
| <I/σ(I)> | 18.45 | 2.56 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 12.4 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M BIS-TRIS PH 5.5 0.6M AMMONIUM ACETATE 20% V/V PEG 3350 |
