3ZEF

Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	125.420, 177.841, 216.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	137.450 - 3.100
R-factor	0.22253
Rwork	0.220
R-free	0.26711
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3SBT 2og4
RMSD bond length	0.011
RMSD bond angle	1.534
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0016)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	137.500	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.160	1.300
Number of reflections	97506
<I/σ(I)>	6.4	1.4
Completeness [%]	99.9	100
Redundancy	5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.9		7-9% PEG 8000, 100 MM NA-CITRATE PH 7.9, 50-200 MM AMMONIUM SULFATE