3WWQ
Crystal structure of FAAP20 UBZ domain in complex with Lys63-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.767, 45.850, 172.900 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.20957 |
| Rwork | 0.208 |
| R-free | 0.23458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.322 |
| Number of reflections | 74073 | |
| <I/σ(I)> | 27 | 4.26 |
| Completeness [%] | 95.1 | 88.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.8M DL-malic acid, 0.1M glycine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
