3WN7
Crystal Structure of Keap1 in Complex with the N-terminal region of the Nrf2 transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.138, 84.394, 69.818 |
| Unit cell angles | 90.00, 116.89, 90.00 |
Refinement procedure
| Resolution | 34.780 - 1.570 |
| R-factor | 0.18923 |
| Rwork | 0.187 |
| R-free | 0.22299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x2j |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.097 | 0.253 |
| Number of reflections | 93450 | |
| <I/σ(I)> | 18.6 | 7.8 |
| Completeness [%] | 98.8 | 88.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 277 | 100mM Sodium HEPES, 0.2M potassium acetate, 20% (w/v) PEG 3350, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
