3W9F
Crystal structure of the ankyrin repeat domain of chicken TRPV4 in complex with IP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-06-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.094, 48.343, 132.145 |
Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
Resolution | 32.807 - 1.900 |
R-factor | 0.2185 |
Rwork | 0.218 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jxi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.086 | 0.619 |
Number of reflections | 100702 | |
<I/σ(I)> | 18.2 | 2.37 |
Completeness [%] | 97.2 | 93.3 |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 110-120 mM sodium MES-HCl buffer pH 6.5, 1-2% PEG 4000, 11-12% MPD, 55-60 mM KH2PO4, vapor diffusion, sitting drop, temperature 293K |