3VOW
Crystal Structure of the Human APOBEC3C having HIV-1 Vif-binding Interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | P 61 |
Unit cell lengths | 105.042, 105.042, 70.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 90.970 - 2.150 |
R-factor | 0.2162 |
Rwork | 0.214 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ir2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 105.050 | 105.050 | 2.190 |
High resolution limit [Å] | 2.150 | 5.840 | 2.150 |
Rmerge | 0.054 | 0.037 | 0.338 |
Number of reflections | 24043 | ||
<I/σ(I)> | 20.1 | ||
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 22.3 | 20.9 | 22.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Bicine, PEG 6000, L-Arginine HCl, pH 9.0, vapor diffusion, hanging drop, temperature 293K |