3VLG

Crystal structure of the W150A mutant LOX-1 CTLD showing impaired OxLDL binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	95
Detector technology	CCD
Collection date	2011-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	62.244, 62.244, 76.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.170 - 2.300
R-factor	0.1824
Rwork	0.179
R-free	0.24648
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yxk
RMSD bond length	0.022
RMSD bond angle	1.815
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.094	0.044	0.369
Number of reflections	6945
<I/σ(I)>	45.81	77.4	10.9
Completeness [%]	97.3	99.3	95.6
Redundancy	13.8	12.2	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	20% PEG8000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K