3VCY
Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.600, 118.510, 92.510 |
| Unit cell angles | 90.00, 116.48, 90.00 |
Refinement procedure
| Resolution | 27.900 - 1.925 |
| R-factor | 0.20215 |
| Rwork | 0.199 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lth |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.482 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.900 |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.126 |
| Number of reflections | 122583 |
| <I/σ(I)> | 4.8 |
| Completeness [%] | 97.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25 %(w/v) PEG 4000, 0.1 M BIS-TRIS pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298 K |
